期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 258, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2021.119805
关键词
Fluorometer; Tryptophan autofluorescence; In-field Sensors; Water pre-screening; Real-time sensor; Early-warning-systems
类别
资金
- RFF Forskningsfond Oslofjordfondet
- Praktisk og effektiv oppflging av KOLS-pasienter i kommunene [285575]
- Lab-on-Fiber: En bio-optisk sensor for deteksjon av ultralav bakteriekonsentrasjon idrikkevannsforsyningsnet [296654]
- Palitelig termisk styringssystem for BEV-batteripakker: intelligent og modulaer losning [293808]
- Chongqing Research Program of Basic Research and Frontier Technology [Cstc2018jcyjax0474, Cstc2018jcyjA3877, cstc2019jcyjmsxmX0776]
- Chongqing Education Commission - Science and Technology Research Program [KJZD-K201800802, KJZD-K201900802, KLZD-K202000805]
This study reports the development and study of an original in-field tryptophan fluorimetric sensor as a potential indicator of real-time microbial contamination in water. The sensor showed good detection sensitivity and linearity in laboratory tests, and exhibited high correlation with coliform bacteria in small-scale in situ tests.
In recent years, global efforts have been directed towards the development of water safety routines, consequently demanding cost-effective sensors capable of detecting outbreaks at early stages. This work reports the development and study of an original in-field tryptophan fluorimetric sensor as a potential indicator of real-time microbial contamination in water. The sensor excitation and emission wavelengths were selected with respect to the coliform bacteria tryptophan peak; 280 nm for excitation and 330 nm for emission. The in-lab tests with standard samples show a detection limit of 4.89 nM (approximate to 0.1 mu g/l) for L-tryptophan. The sensor exhibited good linearity over three orders of magnitude and considerable detection reproducibility, which was confirmed during calibration tests. Small-scale in situ tests showed that the sensor was better correlated with coliform bacteria than other online sensors such as turbidity. This suggests that the fluorimetric tryptophan sensor can be integrated into early warning systems that quickly assess changes in water microbial quality. (C) 2021 Elsevier B.V. All rights reserved.
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