期刊
SOUTH AFRICAN JOURNAL OF BOTANY
卷 141, 期 -, 页码 457-465出版社
ELSEVIER
DOI: 10.1016/j.sajb.2021.06.002
关键词
Barley; Gene expression; Real-time RT-PCR; Salinity
资金
- Center of Excellence in Cereal Molecular Breeding, University of Tabriz, Iran
The study assessed the impact of salinity treatment on barley cultivars, revealing significant effects on plant growth and gene expression. Different cultivars showed varying levels of salt tolerance in response to salt treatment.
Salinity is a crucial limiting factor behind crop production in the world. Understanding the mechanisms of salinity-stress response could contribute to the improvement of salt tolerance in plants. We assessed the effect of salinity treatment (0, 100 and 200 mM), 24 h, 3 days, and 3 weeks after salt exposure on morpholog-ical characters and gene expression pattern of two barley cultivars; Sahara and Clipper and a new Advanced line. The expression pattern of 8 salt-inducible genes namely beta FRUCT3, L1s1, HSP17.8, AOS, SBP, PEAMT, Na+-transporter, and ATLS1) was investigated in barley shoots using the real-time RT-PCR. Plant height, number of leaf/plant, number of tiller/plant, shoot fresh and dry weight and shoot water content were measured. The NaCl treatment significantly affects shoot fresh and dry weight as well as shoot water content and significant differences were observed among genotypes for all the studied traits except shoot dry weight and number of leaf/plant. The expression of the studied genes except ATLS1 was significantly affected by salt treatment, genotype and timepoint exposure to salinity. The mRNA level of beta FRUCT3, L1s1, PEAMT and Na+-transporter genes was up-regulated in the salt-tolerant cultivar Sahara and down-regulated in the salt-sensitive cultivar Clipper after 3 weeks exposure to salt treatment. (C) 2021 SAAB. Published by Elsevier B.V. All rights reserved.
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