Salt-tolerance of Phaseolus vulgaris L. is a function of the potentiation extent of antioxidant enzymes and the expression profiles of polyamine encoding genes

Selection and improvement of crop salt tolerance constitutes an urgent need for increasing agricultural and food production to feed the growing human population. Two bean (Phaseolus vulgaris L.) genotypes, namely Tema and Djadida varying in their tolerance to NaCl, were selected to investigate the determinant physiolog-ical and molecular mechanisms underlying salt tolerance. At the physiological level, salinity resulted in a sig-nificant decrease in growth, water status, stomatal conductance and photosynthesis for both genotypes. The potassium content was higher in the salt-tolerant genotype Tema under normal and saline conditions. Both genotypes had a significant increase in proline, glucose, fructose and sucrose concentrations, but glucose and fructose were differentially higher in the salt-tolerant genotype. While putrescine and spermidine concentra-tions decreased, spermine concentration significantly enhanced under salt stress along with antioxidant enzymes activities namely catalase, glutathione reductase and ascorbate peroxidase. The increase was higher in the salt-tolerant genotype. The expression levels of genes encoding enzymes involved in spermine biosyn-thesis revealed an upregulation of spermine synthase (SPMS) and a downregulation of polyamine oxidase (PAO4). Overall, the difference between genotypes is more quantitative than qualitative; the salt-tolerant genotype Tema displayed a better physiological and molecular response under salinity. By measuring these molecules in salt-stressed plants, several genotypes could be screened for breeding programmes leading to improve salt tolerance in beans. (c) 2021 SAAB. Published by Elsevier B.V. All rights reserved.

Automated summary

The study focused on the selection and improvement of crop salt tolerance using two bean genotypes with varying levels of tolerance to NaCl. It was found that the salt-tolerant genotype Tema exhibited better physiological and molecular responses under salinity stress, making it a more suitable candidate for breeding programs aimed at improving salt tolerance in beans.