期刊
SMALL
卷 17, 期 29, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.202007174
关键词
exosome isolation; exosome separation; microfluidics
类别
资金
- National Natural Science Foundation of China [81973099, 61874099]
Exosomes are small extracellular vesicles secreted by various cells into body fluids and hold great potential for disease diagnosis and therapy. However, isolating exosomes is challenging due to their heterogeneity and the complexity of bio-fluids. Recent advancements in microfluidic technologies offer an efficient system for exosome extraction, showcasing high speeds, portability, and low sample volumes.
Exosomes, a class of small extracellular vesicles (30-150 nm), are secreted by almost all types of cells into virtually all body fluids. These small vesicles are attracting increasing research attention owing to their potential for disease diagnosis and therapy. However, their inherent heterogeneity and the complexity of bio-fluids pose significant challenges for their isolation. Even the gold standard, differential centrifugation, suffers from poor yields and is time-consuming. In this context, recent developments in microfluidic technologies have provided an ideal system for exosome extraction and these devices exhibit some fascinating properties such as high speeds, good portability, and low sample volumes. In this review, the focus is on the state-of-the-art microfluidic technologies for exosome isolation and highlight potential directions for future research and development by analyzing the challenges faced by the current strategies.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据