期刊
SMALL
卷 17, 期 26, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.202101678
关键词
cell apoptosis; DNA origami; FasL; FasR; ligand-receptor interactions; signaling complex
类别
资金
- Deutsche Forschungsgemeinschaft (DFG) Fellowship through the Graduate School of Quantitative Biosciences Munich (QBM)
- Deutsche Forschungsgemeinschaft DFG [SFB1032, SFB1208]
- Emmy Noether program of the Deutsche Forschungsgemeinschaft DFG
- Freigeist fellowship by Volkswagen Foundation
- Projekt DEAL
Cell signaling is initiated by specific protein patterns on the plasma membrane. DNA origami-based nanoagents with precise arrangements of death receptor ligand FasL were introduced to cells, leading to faster and more efficient signaling compared to naturally occurring soluble FasL. This discovery provides unprecedented control over molecular signaling and a new strategy for driving specific cell responses.
Cell signaling is initiated by characteristic protein patterns in the plasma membrane, but tools to decipher their molecular organization and activation are hitherto lacking. Among the well-known signaling pattern is the death inducing signaling complex with a predicted hexagonal receptor architecture. To probe this architecture, DNA origami-based nanoagents with nanometer precise arrangements of the death receptor ligand FasL are introduced and presented to cells. Mimicking different receptor geometries, these nanoagents act as signaling platforms inducing fastest time-to-death kinetics for hexagonal FasL arrangements with 10 nm inter-molecular spacing. Compared to naturally occurring soluble FasL, this trigger is faster and 100x more efficient. Nanoagents with different spacing, lower FasL number or higher coupling flexibility impede signaling. The results present DNA origami as versatile signaling scaffolds exhibiting unprecedented control over molecular number and geometry. They define molecular benchmarks in apoptosis signal initiation and constitute a new strategy to drive particular cell responses.
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