期刊
SEPARATION AND PURIFICATION TECHNOLOGY
卷 265, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.seppur.2021.118476
关键词
Antibody purification; Affinity; Capture; Antibody fragments; synthetic ligands
资金
- Fundacao para a Ciencia e Tecnologia (FCT) [PTDC/BII-BIO/28878/2017]
- ERDF under the PT2020 Partnership Agreement [LISBOA-01-0145FEDER-028878]
- MIT-PT PhD Bioengineering Systems [PD/BD/128251/2016]
- Applied Molecular Biosciences Unit -UCIBIO [UIDP/04378/2020, UIDB/04378/2020]
- CICECO-Aveiro Institute of Materials - FCT [UIDB/50011/2020, UIDP/50011/2020]
- [SFRH/BPD/97585/2013]
- [SFRH/BPD/112543/2015]
- Fundação para a Ciência e a Tecnologia [PTDC/BII-BIO/28878/2017] Funding Source: FCT
Antibody technologies are a successful case in the biopharmaceutical industry, with researchers developing affinity adsorbents inspired by natural and engineered antibody-binding ligands to capture full-length antibodies and fragments in a single-step recovery process.
Antibody technologies are the most representative success-case in the biopharmaceutical industry. Widely available purification technologies fail in providing a dedicated universal purification platform that can accommodate antibodies structural diversity, namely antibodies from non-human sources, as chicken IgY, and antigen-binding fragments. In this work, we took inspiration from natural and engineered antibody-binding ligands, to rationally design affinity adsorbents able to capture full-length antibodies and fragments. The one-pot Petasis and Ugi combinatorial reactions were sequentially employed to rapidly generate a library of putative solid-phase adsorbents. The best performing adsorbent yielded a single-step recovery, under mild conditions, of human and chicken whole antibodies, antigen-binding fragments and engineered single-domain antibodies from different complex feedstocks. Due to its simple preparation, the lead antibody adsorbent finds broad applicability as a universal purification platform to increase the availability of antibody technologies in research and development.
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