期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 341, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2021.130008
关键词
Electrochemiluminescence; Ratio biosensor; Ir nanorods; CdS quantum dots; Organophosphorus pesticides
资金
- National Natural Science Foundation of China [21775122, 21775124]
- Natural Science Foundation of Chongqing City, China [cstc2018jcyjAX0693]
This study prepared water-soluble Ir nanorods and developed a highly sensitive dual-signal nanoprobe for detecting organophosphorus pesticides, utilizing the opposite changes in two ECL signals for target detection.
The poor solubility and limited ECL emission efficiency of iridium (Ir) complexes in aqueous restricted their application in ECL field. This work prepared water-soluble Ir nanorods (Ir NRs) via carboxyl-functionalizing tris (2-phenylpyridine) iridium(III) (Ir(ppy)3) using poly(styrene-co-maleicanhydride) (PSMA) and further developed a highly sensitive dual-signal nanoprobe with Ir NRs as anodic emitter and CdS quantum dots (QDs) as cathodic emitter. Ir NRs@CdS QDs served as the matrix to immobilize enzymes to construct an ECL ratio biosensor for detecting organophosphorus pesticides (OPs). The dual-functional H2O2 produced by enzymatic reaction had an opposite effect on two ECL signals, namely enhanced the cathodic signal and quenched the anodic signal. With the OPs, the activity of enzyme was inhibited and the generation of H2O2 was prevented, thus the opposite changes of two ECL signals were detected. The detection of ethyl paraoxon (EP) as target OPs was achieved with a linear range from 5.0 x 10-12 to 5.0 x 10-8 M and detection limit was 1.67 x 10-12 M. The excellent solubility and high ECL efficiency of Ir NRs in aqueous greatly expanded the application of Ir(III) complexes. The opposite effect of H2O2 on two emissions of Ir NRs@CdS QDs provided a novel ratio ECL platform for bioanalysis.
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