期刊
SCIENCE SIGNALING
卷 14, 期 688, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/scisignal.abe6156
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资金
- Cancer Research UK [A16942, A29580] Funding Source: Medline
- European Research Council [694620] Funding Source: Medline
- Medical Research Council [MC_UU_00002/16] Funding Source: Medline
- European Research Council (ERC) [694620] Funding Source: European Research Council (ERC)
Unlike early transcriptional responses to mitogens, the later events in the response to epidermal growth factor (EGF) are less well understood. This study identified delayed down-regulated genes (DDGs) in mammary cells after prolonged EGF treatment, showing that some DDGs may act as tumor suppressors. The transcription factor TSHZ2, encoded by a DDG, inhibits tumor growth and metastasis and plays a role in cell cycle regulation.
Unlike early transcriptional responses to mitogens, later events are less well-characterized. Here, we identified delayed down-regulated genes (DDGs) in mammary cells after prolonged treatment with epidermal growth factor (EGF). The expression of these DDGs was low in mammary tumors and correlated with prognosis. The proteins encoded by several DDGs directly bind to and inactivate oncoproteins and might therefore act as tumor suppressors. The transcription factor teashirt zinc finger homeobox 2 (TSHZ2) is encoded by a DDG, and we found that overexpression of TSHZ2 inhibited tumor growth and metastasis and accelerated mammary gland development in mice. Although the gene TSHZ2 localizes to a locus (20q13.2) that is frequently amplified in breast cancer, we found that hypermethylation of its promoter correlated with down-regulation of TSHZ2 expression in patients. Yeast two-hybrid screens and protein-fragment complementation assays in mammalian cells indicated that TSHZ2 nucleated a multiprotein complex containing PRC1/Ase1, cyclin B1, and additional proteins that regulate cytokinesis. TSHZ2 increased the inhibitory phosphorylation of PRC1, a key driver of mitosis, mediated by cyclin-dependent kinases. Furthermore, similar to the tumor suppressive transcription factor p53, TSHZ2 inhibited transcription from the PRC1 promoter. By recognizing DDGs as a distinct group in the transcriptional response to EGF, our findings uncover a group of tumor suppressors and reveal a role for TSHZ2 in cell cycle regulation.
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