Pairing denitrifying phosphorus accumulating organisms with anaerobic ammonium oxidizing bacteria for simultaneous N and P removal

Coupling of denitrifying polyphosphate accumulating organisms (DPAO) with anaerobic ammonium oxidizing (Anammox) bacteria in a single treatment scheme has so far been unsuccessful but could offer substantial energy savings, minimize sludge production, while achieving simultaneous carbon, nitrogen and phosphate removal. However, both organisms compete for nitrite and have vastly different growth rates and therefore the goal of this study was to uncouple their solid retention time (SRT) by growing them in different sludge fractions and to determine their biomass specific kinetic properties. Anammox bacteria were grown in a biofilm for longer SRTs and DPAO in flocs to allow shorter SRTs. Exposure of DPAO to anaerobic conditions was accomplished by recycling the flocs to a separate reactor by which simultaneous P, N, and C removal was accomplished. The diffusion limited biofilm lowered the biomass specific nitrite affinity for Anammox (K-s(AMX) = 0.091 mM), which gave DPAO a competitive edge to consume nitrite (K-s(DPAO) = 0.022 mM) in the suspended floc fraction. However, DPAO are more sensitive to nitrite (K-i(DPAO) = 0.377 mM) than Anammox bacteria and (K-i(AMX) > 1.786 mM), and therefore the DPAO would be better suited to grow in the protective biofilm, showing that both biomass growth types (flocs and granules) have advantages (and disadvantages) depending on the setting. This work is an important steppingstone to understanding resource competition amongst Anammox and DPAO and SRT management strategies to allow their pairing in combined reactor configurations. (C) 2021 Elsevier B.V. All rights reserved.

Automated summary

Coupling denitrifying polyphosphate accumulating organisms (DPAO) with anaerobic ammonium oxidizing (Anammox) bacteria in a single treatment scheme has challenges due to their competition for nitrite and different growth rates. This study aimed to uncouple their solid retention time (SRT) by growing them in different sludge fractions and found that Anammox bacteria had higher nitrite affinity in biofilms, while DPAO were more sensitive to nitrite and better suited for growth in the protective biofilm.