期刊
SCIENCE OF THE TOTAL ENVIRONMENT
卷 774, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.scitotenv.2021.145766
关键词
Embryonic hatching inhibition; Co-exposure; Microcystins-LR; Polystyrene nanoplastics; Mechanisms
资金
- National Key Research and Development Program of China [2019YFD0900602]
- Fundamental Research funds for the Central Universities [2662018PY061]
- General program of Natural Science Foundation of Hubei Province [2019CFB771]
The study found that the presence of PSNPs exacerbated the inhibitory effects of MCLR on the hatching of F1 zebrafish embryos, by affecting enzyme activity, interfering with the cholinergic system, and impacting muscle development.
The combined toxicity effects of microcystins-LR (MCLR) and polystyrene nanoplastics (PSNPs) on the hatching of F1 zebrafish (Danio rerio) embryos were investigated in this study due to the increasing concerns of both plastic pollution and eutrophication in aquatic environments. Three-month-old zebrafish were used to explore the molecular mechanisms underlying the combined effect of MCLR (0, 0.9, 4.5, and 22.5 mu g/L) on egg hatching in the existence of PSNPs (100 mu g/L). The results demonstrated the existence of PSNPs further increased the accumulation of MCLR in F1 embryos. The hatching rates of F1 embryos were inhibited after exposure to 22.5 mu g/L MCLR, and the presence of PSNPs aggravated the hatching inhibition induced by MCLR. The decrease of hatching enzyme activity and the abnormality of spontaneous movement were observed. We examined the altered expression levels of the genes associated with the hatching enzyme (tox16, foxp1, ctslb, xpb1, klf4, cap1, bmp4, cd63, He1.2, zhe1, and prl), cholinergic system (ache and chrn alpha 7), and muscle development (Wnt, MyoD, Myf5, Myogenin, and MRF4). The results suggested the existence of PSNPs exacerbated the hatching inhibition of F1 embryos through decreasing the activity of enzyme, interfering with the cholinergic system, and affecting the muscle development. (C) 2021 Published by Elsevier B.V.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据