期刊
SCIENCE OF THE TOTAL ENVIRONMENT
卷 806, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.scitotenv.2021.150488
关键词
Gentamicin mycelial residues; Anaerobic co-digestion; Methanosaeta; Methanogenic pathway; Antibiotic resistance genes
资金
- National Natural Science Foundation of China [51978497]
- National Key Research and Development Program of China [2018YFC1900905]
The study found that AcoD of GMRs and WS at the appropriate mixing ratio can enhance methane production, reduce the accumulation of toxic substances, maintain system stability, and mitigate the dissemination risks of antibiotic resistance genes.
Anaerobic co-digestion (AcoD) of gentamicin mycelial residues (GMRs), a kind of nitrogen-rich biowaste, and wheat straw (WS) is an attractive technology for the recycling of GMRs. However, the effects of the co substrate ratio on methane production, system stability and antimicrobial resistance during co-digestion remain unclear. Thus, this study aimed to fill in the blanks through AcoD of GMRs and WS with different mixing ratios (1:0, 2:1, 1:1, 1:2, 0:1, VS basis) via batch tests. Results showed that AcoD facilitated methane production than mono anaerobic digestion and reduced the accumulation of the toxic substances, such as ammonia nitrogen and humic-like substances. The maximum methane production was obtained at the reactors with the mixing ratio of 1:1 and 1:2 (R-1:1 and R-1:2), which matched with the relative abundance of key enzymes related to methanogenesis predicted by PICRUSt. Microbial community analysis indicated that Methanosaeta was the most dominant methanogen in the AcoD reactors. The highest relative abundance of Methanosaeta (45.1%) was obtained at R-1:1 due to the appropriate AcoD conditions, thus, providing greater possibilities for high stability of AcoD system. Additionally, AcoD of the GMRs and WS under the mixing ratio of 1:1 and 1:2 did not prompt the increase of antibiotic resistance genes (ARGs). Not only that, the likelihood of horizontal gene transfer declined in R-1:1 due to the weaker connection and transport between host and recipient bacteria. Findings of this study suggested that the suitable mixing ratio of GMRs and WS contributes to methane production and system stability, and reduces the dissemination risks of ARGs. (c) 2021 Published by Elsevier B.V.
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