期刊
SCIENCE OF THE TOTAL ENVIRONMENT
卷 775, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.scitotenv.2021.145671
关键词
Neurotoxicity; Predicted target profile; Acetylcholinesterase inhibitor; Endocrine disruptors; Androgenic effects; Risk assessment
资金
- Spanish Government [PRE2018-083513]
- FEDER Funds [CTM2017-83242-R]
- network of recognized research groups by the Catalan Government [2017 SGR_902]
- Beatriu de Pinos programme by the Secretariat of Universities and Research department of the Ministry for Business and Knowledge, Catalonia Government [2016 BP 00233]
- European Social Fund (ESF)
- Sicily region [24/2018]
- FSE 2014-2020
The study demonstrates that exposure to environmental concentrations of Fenitrothion can induce neurotoxic effects in zebrafish larvae, impacting their basal locomotor activity, visual-motor response, and acoustic/vibrational escape response. Significant non-monotonic concentration-response relationships were observed for Fenitrothion and the expression of certain genes. Furthermore, potential protein targets of Fenitrothion were identified, with interactions with acetylcholinesterase, monoamine-oxidases, and androgen receptor being experimentally validated, suggesting an environmental risk for fish communities.
Fenitrothion is an organophosphorus insecticide usually found in aquatic ecosystems at concentrations in the range of low ng/L. In this manuscript we show that 24 h exposure to environmental concentrations of fenitrothion, from ng/L to low mu g/L, altered basal locomotor activity, visual-motor response and acoustic/vibrational escape response of zebrafish larvae. Furthermore, fenitrothion and expression of gap43a, gfap, atp2b1a, and mbp exhibited a significant non-monotonic concentration-response relationship. Once determined that environmental concentrations of fenitrothion were neurotoxic for zebrafish larvae, a computational analysis identified potential protein targets of this compound. Some of the predictions, including interactions with acetylcholinesterase, monoamine-oxidases and androgen receptor (AR), were experimentally validated. Binding to AR was the most suitable candidate for molecular initiating event, as indicated by both the up-regulation of cyp19a1b and sult2st3 and the non-monotonic relationship found between fenitrothion and the observed responses. Finally, when the integrity of the monoaminergic system was evaluated, altered levels of L-DOPA, DOPAC, HVA and 5-HIAA were found, as well as a significant up-regulation of slc18a2 expression at the lowest concentrations of fenitrothion. These data strongly suggest that concentrations of fenitrothion commonly found in aquatic ecosystems present a significant environmental risk for fish communities. (C) 2021 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
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