期刊
SCIENCE CHINA-LIFE SCIENCES
卷 65, 期 2, 页码 376-386出版社
SCIENCE PRESS
DOI: 10.1007/s11427-020-1908-2
关键词
circSVIL; STAT1; skeletal muscle
类别
资金
- National Natural Science Foundation of China [31772574]
Circular RNA circSVIL promotes bovine myoblast proliferation and inhibits apoptosis, by interacting with STAT1 to inhibit its phosphorylation and affect downstream signaling cascade.
Circular RNAs (circRNAs), a novel class of non-coding RNAs with a loop structure, have recently been shown to participate in various pathophysiological processes. However, the precise role of circRNAs in myoblasts remains unclear. In this report, circSVIL was screened and identified from our previous sequencing analysis; we then performed gain- and loss-of-function experiments on bovine myoblasts by CCK8, EdU, flow cytometry, qRT-PCR, and Western blotting. The results indicate that circSVIL facilitates bovine myoblast proliferation and inhibits cell apoptosis. Using mechanism assays such as bioinformatics prediction, RNA immunoprecipitation (RIP), and cytoplasmic separation, we demonstrate that circSVIL could interact with STAT1 and inhibit STAT1 phosphorylation, thereby restraining STAT1's nuclear translocation and affecting its downstream signal cascade. Our results may elucidate a new regulatory pathway for bovine skeletal muscle development.
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