期刊
SCIENCE CHINA-LIFE SCIENCES
卷 65, 期 5, 页码 940-952出版社
SCIENCE PRESS
DOI: 10.1007/s11427-021-1948-1
关键词
autophagy; single-cell; RNA-seq; methylation; early human embryo
类别
资金
- National Key Research and Development Program [2019YFA0801400, 2017YFA0103801, 2018YFC1004000]
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDA16020703]
Autophagy plays a crucial role in early human embryo development, with ATG genes showing stable expression patterns during early developmental stages, indicating the continuous activation of the autophagy pathway in early human embryo development.
Early human embryogenesis is a very sophisticated process due to its unique gene regulatory network. Autophagy has been suggested to play an important role in mediating the development of early embryonic cells in mammals. However, evidence showing how autophagy regulates early human embryogenesis remains to be further explored. In this study, we systematically investigated the human transcriptome and methylome patterns of autophagy-related (ATG) genes in early embryonic cells at single-cell resolution. We analyzed the transcriptomic data of 365 cells and methylome data of 265 cells. The results showed that most ATG genes remained epigenetically active and were expressed stably throughout early embryogenesis, whereas the dynamics varied among different developmental stages. This evidence indicated that the autophagy pathway was constitutively activated and exerted a fundamental role in early human embryo development. Our work, for the first time, comprehensively reveals the features of autophagy during early human embryo development.
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