期刊
RNA
卷 27, 期 9, 页码 1004-1016出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1261/rna.078709.121
关键词
RNA interference; TNRC6; GW182; alternative splicing; Argonaute; RNA sequencing
资金
- National Institutes of Health (NIH) [GM106151, 5 F31 EY030336-03]
- Robert Welch Foundation [I-1244]
The TNRC6 paralogs have largely redundant functions in RNA-mediated control of gene expression, showing high correlation with AGO paralogs. They play roles in the RNA interference machinery.
TNRC6 is a scaffolding protein that bridges interactions between small RNAs, argonaute (AGO) protein, and effector proteins to control gene expression. There are three paralogs in mammalian cells, TNRC6A, TNRC6B, and TNRC6C. These paralogs have similar to 40% amino acid sequence identity and the extent of their unique or redundant functions is unclear. Here, we use knockout cell lines, enhanced crosslinking immunoprecipitation (eCLIP), and high-throughput RNA sequencing (RNA-seq) to explore the roles of TNRC6 paralogs in RNA-mediated control of gene expression. We find that the paralogs are largely functionally redundant and changes in levels of gene expression are well-correlated with those observed in AGO knockout cell lines. Splicing changes observed in AGO knockout cell lines are also observed in TNRC6 knockout cells. These data further define the roles of the TNRC6 isoforms as part of the RNA interference (RNAi) machinery.
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