期刊
REPRODUCTIVE SCIENCES
卷 29, 期 3, 页码 975-992出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s43032-021-00710-3
关键词
E-cadherin; N-cadherin; SLUG; Glycogen synthase kinase (GSK)-3; MicroRNA (miR)-149; Poly(ADP-ribose) polymerase-2 (PARP-2); Caspase-8; Endometrial receptivity; Pseudopregnancy; Endometrial receptivity failure
资金
- Indian Council of Medical Research (ICMR), New Delhi [5/10/10/2011-RHN]
- DBT, New Delhi [BT/PR30749/MED/97/421/2018]
- Science and Engineering Research Board (SERB), Department of Science and Technology (DST), New Delhi, India [EMR/2016/005278-2018]
E-cadherin is predominantly expressed during the pre-receptive stage of endometrial receptivity and is promoted by PARP-2, which is regulated by miR-149. On the other hand, N-cadherin is only detected at the early stages and decreases thereafter.
Cadherins play an essential role in the attachment of the blastocyst to the endometrium, a process known as endometrial receptivity. Loss of E-cadherin expression is essential during the process, while the expression level of the other cadherin, N-cadherin, has been reported to be altered in cases of infertility. Both E-cadherin and N-cadherin can be regulated by members of the PARP family. Specifically, PARP-2, which is under the epigenetic control of miR-149, has been observed to promote E-cadherin expression in other human cells. We investigated the roles of E-cadherin and N-cadherin in endometrial receptivity using mouse models for normal endometrial receptivity, pseudopregnancy, and LPS-induced endometrial receptivity failure. E-cadherin and phosphorylated E-cadherin were predominantly expressed during pre-receptive stages as well as in the implantation site of the receptive stage, which were observed reduced during the later stages of implantation in both implantation and non-implantation regions, while N-cadherin was detected only at pre-receptive stages. E-cadherin and N-cadherin were also seen in the uterus during pseudopregnancy, showing a downregulation trend during receptive and post-receptive stages. LPS-induced failed endometrial receptivity showed upregulation of E-cadherin and downregulation of N-cadherin. The E-cadherin expression promoter, GSK-3, was lost and its suppressor, SLUG was upregulated during normal course of endometrial receptivity in mouse model, while GSK-3 was increased during LPS-induced failed embryo implantation. In an in vitro model of embryo implantation, E-cadherin expression is promoted by PARP-2 and regulated by miR-149 epigenetically in human endometrium epithelial cells. In conclusion, E-cadherin is predominantly expressed during pre-receptive stage and promoted by PARP-2, which is regulated by miR-149 in the endometrial epithelial cells.
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