4.5 Article

Identification of Adrenomedullin-Induced S-Nitrosylated Proteins in JEG-3 Placental Cells

期刊

REPRODUCTIVE SCIENCES
卷 29, 期 4, 页码 1296-1304

出版社

SPRINGER HEIDELBERG
DOI: 10.1007/s43032-021-00663-7

关键词

ADM-induced S-nitrosylated proteins; ANX II; Adrenomedullin; Human extravillous cytotrophoblast; Invasion

资金

  1. National Key Research and Development Program of China [2018YFC1004104]

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This study identified several proteins S-nitrosylated by ADM in JEG-3 placental cells, including tubulin, enolase, eukaryotic translation initiation factor 4A1, actin, ANX II, and glyceraldehyde 3-phosphate dehydrogenaseprotein-1. ADM significantly increased the surface expression of ANX II, but not its total expression, in JEG-3 cells, which could potentially impact trophoblast invasion and migration. Future studies are needed to further understand the roles of S-nitrosylated ANX II in trophoblast functions.
Extravillous cytotrophoblast (EVCT) is responsible for trophoblast invasion, which is important during placentation. Dysregulation of the process leads to pregnancy complications. S-nitrosylation of proteins is associated with cell invasion in many cell types. Adrenomedullin (ADM), a polypeptide expressed abundantly in the first-trimester placentas, induces EVCT invasion by upregulation of protein S-nitrosylation. This study aimed to identify the S-nitrosylated proteins induced by ADM in the JEG-3 placental cells. By using affinity chromatography followed by mass spectrometric analysis, tubulin, enolase, eukaryotic translation initiation factor 4A1, actin, annexin II (ANX II), and glyceraldehyde 3-phosphate dehydrogenaseprotein-1 were found to be S-nitrosylated by ADM. In vitro treatment with ADM or S-Nitrosoglutathione (GSNO) significantly increased the ANX II surface expression, but not its total expression in the JEG-3 cells. Translocation of ANX II to cell surface has been reported to act as a cell surface receptor to plasmin, plasminogen, and tissue plasminogen activator (tPA), thereby stimulating cell invasion and migration. However, in this study, ADM-induced surface expression of ANX II in the JEG-3 cells was not associated with changes in the secretory and membrane-bound tPA activities. Future studies are required to understand the roles of surface expression of S-nitrosylated ANX II on trophoblast functions. To conclude, this study provided evidences that ADM regulated the nitric oxide signaling pathway and modulated trophoblast invasion.

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