期刊
RENEWABLE ENERGY
卷 174, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.renene.2021.04.107
关键词
Cellulases; Xylanases; Biomass saccharification; Trichoderma reesei; Miscanthus; Bioenergy crops
资金
- project of Huazhong Agricultural University Independent Scientific & Technological Innovation Foundation [2662019PY054, 2662020ZKPY013]
- National 111 Project of Ministry of Education of China [BP0820035]
- Project of Hubei University of Arts Science [XKQ2018006]
This study demonstrated that using a specific Miscanthus substrate to induce Trichoderma reesei can enhance enzymatic saccharification efficiency of various lignocellulose residues, particularly for hemicellulose hydrolysis. The induced enzymes consist of distinct cellulases and high-activity xylanases, providing a potential strategy for large-scale biofuel production in major bioenergy crops.
Bioenergy crops provide enormous renewable biomass resources convertible for biofuel production, but lignocellulose recalcitrance fundamentally determines its enzymatic saccharification at high cost and low efficiency. In this study, total 30 diverse Miscanthus lignocellulose substrates were incubated with T. reesei strain to secret lignocellulose-degradation enzymes, and their major wall polymers features (cellulose crystallinity, hemicellulose arabinose and lignin H-monomer) were meanwhile examined with distinct impacts on the enzyme activities. Using characteristic Miscanthus (Msi62) de-lignin residue as inducing substrate with the reesei strain, this study detected that the Msi62-induced enzymes were of consistently higher enhancements on enzymatic saccharification of various lignocellulose residues examined in 17 grassy and woody bioenergy crops, particularly for the hemicellulose hydrolyses, compared to other two reesei-secreted cellulases and three commercial enzymes. Notably, based on SDSgel protein separation profiling and LC-MS/MS analysis, the Msi62-induced enzymes consist of distinct cellulases (CBHI, BG, EGII) compositions and high-activity xylanases. Therefore, this study has demonstrated an applicable approach to achieve the optimal cellulases and xylanases cocktails that enable for low-costly and high-efficient enzymatic saccharification of diverse lignocellulose sources, providing a potential strategy for large-scale biofuel production in all major bioenergy crops. (c) 2021 Elsevier Ltd. All rights reserved.
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