Degradation and biological performance of porous osteomimetic biphasic calcium phosphate in vitro and in vivo

The combination between biphasic calcium phosphate (BCP) and the osteomimetic porous micro-structure obtained via freeze casting is hoped to achieve excellent bone regeneration, while the effects of HA and beta-TCP ratio changes on the degradation and biological performance of the BCP scaffolds with this unique micro-structure need to be determined. Here, we prepared the osteomimetic BCP scaffolds with different HA/beta-TCP ratios (HA30/beta-TCP70, HA50/beta-TCP50, HA70/beta-TCP30) and the effects of different HA/beta-TCPHA/beta-TCP ratios on the degradation and biological performance were studied in vitro and vivo. These BCP scaffolds with different HA/beta-TCP ratios exhibited similar microstructure, mechanical performance, and protein absorption capability, while HA70/beta-TCP30 BCP scaffolds showed an advisable degradation rate. Study in vitro confirmed the bio-compatibility and promotion on the proliferation, differentiation of MG63 cells in the porous osteomimetic BCP scaffolds with a HA/beta-TCP ratio at 30:70. Implantation experiments also showed that the porous osteomimetic BCP scaffolds with a HA/beta-TCP ratio at 30:70 had excellent bone regeneration capacity and proper degradation rate compatible with bone growth. These results reveal that the porous osteomimetic BCP scaffold with a HA/beta-TCP ratio at 30:70 is a potential candidate of biodegradable bone substitutes used for bone repair.

Automated summary

The combination of biphasic calcium phosphate (BCP) with osteomimetic porous micro-structure obtained via freeze casting is believed to achieve excellent bone regeneration, while the effects of different ratios of HA and beta-TCP on the degradation and biological performance of the BCP scaffolds need to be studied. The BCP scaffolds with varying HA/beta-TCP ratios demonstrated similar microstructure, mechanical performance, and protein absorption capacity, with the HA70/beta-TCP30 ratio showing optimal degradation rate. The in vitro and in vivo experiments confirmed the biocompatibility and bone regeneration capacity of the porous osteomimetic BCP scaffold with a 30:70 HA/beta-TCP ratio.