期刊
PROTEIN AND PEPTIDE LETTERS
卷 28, 期 10, 页码 1083-1089出版社
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/0929866528666210720142359
关键词
Phytic acid; phytase; Bacillus subtilis subsp; subtilis JJBS250; solid state; fermentation; wheat bran; dephytinization
资金
- Department of Science and Technology, New Delhi [SR/FT/LS-95/2010]
This study focused on optimizing phytase production by Bacillus subtilis subsp. subtilis JJBS250 in solid state fermentation and its application in dephytinization of food samples. Results showed a significant improvement in phytase production under optimized conditions, leading to the release of nutritional components in food samples.
Background: Phytic acid acts as anti-nutritional factor in food and feed ingredients for monogastric animals as they lack phytases. Objective: Phytase production by Bacillus subtilis subsp. subtilis JJBS250 was studied in solid-s-tate fermentation and its applicability in dephytinization of food. Methods: Bacterial culture was grown in solid state fermentation using wheat bran and various cul-ture conditions were optimized using 'One variable at a time' (OVAT) approach. Effects of differ-ent substrates (wheat bran, wheat straw, sugarcane bagasse), incubation time (24, 48, 72 and 96 h), incubation temperatures (25, 30, 35 and 40oc), pH (4.0, 5.0, 6.0, 7.0 and 8.0) and moisture content (1:1.5, 1:2.0, 1:2.5 and 1:3) were studied on phytase production. Bacterial phytase was used in de-phytinization of food samples. Results: Optimization of phytase production was studied in solid state fermentation (SSF) using 'One variable at a time' (OVAT) approach. Bacillus subtilis subsp. subtilis JJBS250 grew well in various agroresidues in SSF and secreted high enzyme titres using wheat bran at 30oc and pH 5.0 after incubation time of 48 h with substrate to moisture ratio of 1:3. Glucose and ammonium sul-phate supplementation to wheat bran further enhanced phytase production in SSF. Optimization of phytase production resulted in 2.4-fold improvement in phytase production in solid state fermenta-tion. The enzyme resulted in dephytinization of wheat and rice flours with concomitant release of inorganic phosphate, reducing sugar and soluble protein. Conclusion: Optimization resulted in 2.34-fold enhancement in phytase production by bacterial culture that showed dephytinization of food ingredients with concomitant release of nutritional components. Therefore, phytase of B. subtilis subsp. subtilis JJBS250 could find application in im-proving nutritional quality of food and feed of monogastric animals.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据