期刊
出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2025769118
关键词
innate immunity; RNA modifications; host-pathogen interactions; nonsegmented negative-sense RNA virus; rhabdovirus
资金
- NIH [F31AI138448, T32AI007245, AI059371, DP2AG055947, R21HG010066]
The host cell N6adenosine mRNA cap methylase PCIF1 attenuates antiviral responses to viruses like VSV by modifying mRNA cap structures. This modification does not affect mRNA stability, translation or viral infectivity, but it weakens the antiviral effects of interferon-beta treatment. The function of PCIF1 and cap-proximal m6Am in host response attenuation likely extends to other viruses such as rabies and measles.
Interferons induce cell-intrinsic responses associated with resistance to viral infection. To overcome the suppressive action of interferons and their effectors, viruses have evolved diverse mechanisms. Using vesicular stomatitis virus (VSV), we report that the host cell N6adenosine messenger RNA (mRNA) cap methylase, phosphorylated C-terminal domain interacting factor 1 (PCIF1), attenuates the antiviral response. We employed cell-based and in vitro biochemical assays to demonstrate that PCIF1 efficiently modifies VSV mRNA cap structures to m7Gpppm6Am and define the substrate requirements for this modification. Functional assays revealed that the PCIF1-dependent modification of VSV mRNA cap structures is inert with regard to mRNA stability, translation, and viral infectivity but attenuates the antiviral effects of the treatment of cells with interferon-beta. Cells lacking PCIF1 or expressing a catalytically inactive PCIF1 exhibit an augmented inhibition of viral replication and gene expression following interferon-beta treatment. We further demonstrate that the mRNA cap structures of rabies and measles viruses are also modified by PCIF1 to m7Gpppm6Am. This work identifies a function of PCIF1 and cap-proximal m6Am in attenuation of the host response to VSV infection that likely extends to other viruses.
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