期刊
出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2019229118
关键词
methane; aerobic; methylamine; glycine betaine; bacteria
资金
- NSF [EAR-1529461]
- Montana Agricultural Experiment Station [911310]
- NASA [80NSSC21K0487]
- Montana Institute on Ecosystems NSF Established Program to Stimulate Competitive Research Program Grant [EPS-1101342]
- NSF Systems and Synthetic Biology program [MCB-1817428]
- Department of Energy (DOE) Joint Genome Institute (JGI) Synthetic Biology Program [504607]
- US DOE JGI, a DOE Office of Science User Facility [DE-AC02-05CH11231]
- M.J. Murdock Charitable Trust
- National Institute of General Medical Sciences of the NIH [P20GM103474]
Reports of biogenic methane synthesis associated with various organisms have accumulated, but understanding of the process at the genetic and enzyme levels remains limited. The methane paradox of CH4 supersaturation in oxic surface waters in marine and freshwater environments challenges the traditional view of strictly anaerobic CH4 synthesis. Recent interest in the phenomenon highlights the importance of comprehending sources and sinks of this potent greenhouse gas.
Reports of biogenic methane (CH4) synthesis associated with a range of organisms have steadily accumulated in the literature. This has not happened without controversy and in most cases the process is poorly understood at the gene and enzyme levels. In marine and freshwater environments, CH4 supersaturation of oxic surface waters has been termed the methane paradox because biological CH4 synthesis is viewed to be a strictly anaerobic process carried out by O2-sensitive methanogens. Interest in this phenomenon has surged within the past decade because of the importance of understanding sources and sinks of this potent greenhouse gas. In our work on Yellowstone Lake in Yellowstone National Park, we demonstrate microbiological conversion of methylamine to CH4 and isolate and characterize an Acidovorax sp. capable of this activity. Furthermore, we identify and clone a gene critical to this process (encodes pyridoxylamine phosphate-dependent aspartate aminotransferase) and demonstrate that this property can be transferred to Escherichia coli with this gene and will occur as a purified enzyme. This previously unrecognized process sheds light on environmental cycling of CH4, suggesting that O2-insensitive, ecologically relevant aerobic CH4 synthesis is likely of widespread distribution in the environment and should be considered in CH4 modeling efforts.
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