4.8 Article

Differential ligand-selective control of opposing enzymatic activities within a bifunctional c-di-GMP enzyme

出版社

NATL ACAD SCIENCES
DOI: 10.1073/pnas.2100657118

关键词

bifunctional enzyme; cyclic di-GMP; heme sensor

资金

  1. NSF [CHE1352040, CHE2003350, 1745025]
  2. Frasch Foundation [824-H17]
  3. NSF
  4. NIH/National Institute of General Medical Sciences (NIGMS) via NSF [DMR-1829070]
  5. NIH/NIGMS [GM-124166]

向作者/读者索取更多资源

Cyclic dimeric guanosine monophosphate (c-di-GMP) acts as a second messenger in bacteria, modulating processes like biofilm formation. A globin-coupled sensor protein (DcpG) was found to have bifunctional c-di-GMP enzymatic activity, with different regulation of GGDEF and EAL domains by gas binding to the heme. DcpG's ability to control enzymatic activity in response to ligand binding is due to the unique properties of the globin domain.
Cyclic dimeric guanosine monophosphate (c-di-GMP) serves as a second messenger that modulates bacterial cellular processes, including biofilm formation. While proteins containing both cdi-GMP synthesizing (GGDEF) and c-di-GMP hydrolyzing (EAL) domains are widely predicted in bacterial genomes, it is poorly understood how domains with opposing enzymatic activity are regulated within a single polypeptide. Herein, we report the characterization of a globin-coupled sensor protein (GCS) from Paenibacillus dendritiformis (DcpG) with bifunctional c-di-GMP enzymatic activity. DcpG contains a regulatory sensor globin domain linked to diguanylate cyclase (GGDEF) and phosphodiesterase (EAL) domains that are differentially regulated by gas binding to the heme; GGDEF domain activity is activated by the Fe(II)-NO state of the globin domain, while EAL domain activity is activated by the Fe(II)-O2 state. The in vitro activity of DcpG is mimicked in vivo by the biofilm formation of P. dendritiformis in response to gaseous environment, with nitric oxide conditions leading to the greatest amount of biofilm formation. The ability of DcpG to differentially control GGDEF and EAL domain activity in response to ligand binding is likely due to the unusual properties of the globin domain, including rapid ligand dissociation rates and high midpoint potentials. Using structural information from small-angle X-ray scattering and negative stain electron microscopy studies, we developed a structural model of DcpG, providing information about the regulatory mechanism. These studies provide information about full-length GCS protein architecture and insight into the mechanism by which a single regulatory domain can selectively control output domains with opposing enzymatic activities.

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