期刊
出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2105288118
关键词
coxsackievirus replication speed fitness capsid
资金
- NIH National Institute of Allergy and Infectious Disease (NIAID) [R01AI74668, R01AI158351]
- Burroughs Wellcome Fund Investigators in the Pathogenesis of Infectious Diseases Award
- Howard Hughes Medical Institute
- NIH [R01AI74668-13S1, T32GM109776]
RNA viruses can mutate to increase replication speed in cultured cells, but this may come at the cost of reduced stability and fitness. Selecting for the earliest viral progeny can reveal unexpected inefficiencies in the viral replication cycle.
RNA viruses exist as genetically heterogeneous populations due to high mutation rates, and many of these mutations reduce fitness and/or replication speed. However, it is unknown whether mutations can increase replication speed of a virus already well adapted to replication in cultured cells. By sequentially passaging coxsackievirus B3 in cultured cells and collecting the very earliest progeny, we selected for increased replication speed. We found that a single mutation in a viral capsid protein, VP1-F106L, was sufficient for the fast-replication phenotype. Characterization of this mutant revealed quicker genome release during entry compared to wild-type virus, highlighting a previously unappreciated infection barrier. However, this mutation also reduced capsid stability in vitro and reduced replication and pathogenesis in mice. These results reveal a tradeoff between overall replication speed and fitness. Importantly, this approach-selecting for the earliest viral progeny-could be applied to a variety of viral systems and has the potential to reveal unanticipated inefficiencies in viral replication cycles.
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