Characterization of E93 in neometabolous thrips Frankliniella occidentalis and Haplothrips brevitubus

Insect metamorphosis into an adult occurs after the juvenile hormone (JH) titer decreases at the end of the juvenile stage. This generally coincides with decreased transcript levels of JH-response transcription factors Kruppel homolog 1 (Kr-h1) and broad (br), and increased transcript levels of the adult specifier E93. Thrips (Thysanoptera) develop through inactive and non-feeding stages referred to as propupa and pupa, and this type of distinctive metamorphosis is called neometaboly. To understand the mechanisms of hormonal regulation in thrips metamorphosis, we previously analyzed the transcript levels of Kr-h1 and br in two thrips species, Frankliniella occidentalis (Thripidae) and Haplothrips brevitubus (Phlaeothripidae). In both species, the transcript levels of Kr-h1 and br decreased in the propupal and pupal stages, and their transcription was upregulated by exogenous JH mimic treatment. Here we analyzed the developmental profiles of E93 in these two thrips species. Quantitative RT-PCR revealed that E93 expression started to increase at the end of the larval stage in F. occidentalis and in the propupal stage of H. brevitubus, as Kr-h1 and br mRNA levels decreased. Treatment with an exogenous JH mimic at the onset of metamorphosis prevented pupal-adult transition and caused repression of E93. These results indicated that E93 is involved in adult differentiation after JH titer decreases at the end of the larval stage of thrips. By comparing the expression profiles of Kr-h1, br, and E93 among insect species, we propose that the propupal and pupal stages of thrips have some similarities with the holometabolous prepupal and pupal stages, respectively.

Automated summary

In thrips metamorphosis, the adult specifier gene E93 starts to increase in expression at the end of the larval stage or in the propupal stage, coinciding with decreased levels of Kruppel homolog 1 and broad genes. Exogenous treatment with a JH mimic can prevent pupal-adult transition and repress the expression of E93. Comparing the expression profiles of key genes among different insect species suggests similarities between thrips' propupal and pupal stages with holometabolous species.