A Genome-Wide Analysis of Antibiotic Producing Genes in Streptomyces globisporus SP6C4

Soil is the major source of plant-associated microbes. Several fungal and bacterial species live within plant tissues. Actinomycetes are well known for producing a variety of antibiotics, and they contribute to improving plant health. In our previous report, Streptomyces globisporus SP6C4 colonized plant tissues and was able to move to other tissues from the initially colonized ones. This strain has excellent antifungal and antibacterial activities and provides a suppressive effect upon various plant diseases. Here, we report the genome-wide analysis of antibiotic producing genes in S. globisporus SP6C4. A total of 15 secondary metabolite biosynthetic gene clusters were predicted using antiSMASH. We used the CRISPR/Cas9 mutagenesis system, and each biosynthetic gene was predicted via protein basic local alignment search tool (BLAST) and rapid annotation using subsystems technology (RAST) server. Three gene clusters were shown to exhibit antifungal or antibacterial activity, viz. cluster 16 (lasso peptide), cluster 17 (thiopeptide-lantipeptide), and cluster 20 (lantipeptide). The results of the current study showed that SP6C4 has a variety of antimicrobial activities, and this strain is beneficial in agriculture.

Automated summary

Soil serves as a major reservoir for plant-associated microbes, with actinomycetes playing a crucial role in producing antibiotics that contribute to plant health. The study conducted a genome-wide analysis of antibiotic producing genes in Streptomyces globisporus SP6C4, revealing 15 secondary metabolite biosynthetic gene clusters. Three of these gene clusters were found to exhibit antifungal or antibacterial activity, showcasing the potential benefits of SP6C4 in agriculture.