期刊
PLANT JOURNAL
卷 107, 期 5, 页码 1283-1298出版社
WILEY
DOI: 10.1111/tpj.15417
关键词
polyamines; cadaverine; biotin; root architecture; Arabidopsis thaliana
资金
- College of Agriculture and Life Sciences [WIS10338]
- Office of the Vice Chancellor for Research and Graduate Education, University of Wisconsin-Madison [MSN226688]
- National Aeronautics and Space Administration [80NSSC19K1483]
- University of Wisconsin-Madison
- NSF [BIR-9512577]
- NIH [S10RR13790]
- National Science Foundation [EPS 0236913, MCB 1413036, DBI 0521587, DBI1228622]
- Kansas Technology Enterprise Corporation
- K-IDeA Networks of Biomedical Research Excellence (INBRE) of National Institute of Health [P20GM103418]
- Kansas State University
The study identified the important impact of cadaverine on plant root growth, potentially through modulation of biotin biosynthesis. The molecular mechanisms underlying root growth regulation by cadaverine require further investigation.
Cadaverine, a polyamine, has been linked to modification of root growth architecture and response to environmental stresses in plants. However, the molecular mechanisms that govern the regulation of root growth by cadaverine are largely unexplored. Here we conducted a forward genetic screen and isolated a mutation, cadaverine hypersensitive 3 (cdh3), which resulted in increased root-growth sensitivity to cadaverine, but not other polyamines. This mutation affects the BIO3-BIO1 biotin biosynthesis gene. Exogenous supply of biotin and a pathway intermediate downstream of BIO1, 7,8-diaminopelargonic acid, suppressed this cadaverine sensitivity phenotype. An in vitro enzyme assay showed cadaverine inhibits the BIO3-BIO1 activity. Furthermore, cadaverine-treated seedlings displayed reduced biotinylation of Biotin Carboxyl Carrier Protein 1 of the acetyl-coenzyme A carboxylase complex involved in de novo fatty acid biosynthesis, resulting in decreased accumulation of triacylglycerides. Taken together, these results revealed an unexpected role of cadaverine in the regulation of biotin biosynthesis, which leads to modulation of primary root growth of plants.
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