期刊
PLANT JOURNAL
卷 108, 期 2, 页码 347-357出版社
WILEY
DOI: 10.1111/tpj.15441
关键词
DNA methylation; RNA-directed DNA methylation; small RNAs; WD40; FVE; MSI4
资金
- National Natural Science Foundation of China [NSFC 31900482]
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDB27040000]
Research indicates that FVE plays a key role in DNA methylation at thousands of RdDM target regions and reduces the accumulation of 24-nucleotide siRNA. By physically interacting, FVE may function together with the downstream factor RDM15 in the RdDM pathway.
DNA methylation is an important epigenetic mark. In plants, de novo DNA methylation occurs mainly through the RNA-directed DNA methylation (RdDM) pathway. Researchers have previously inferred that a flowering regulator, MULTICOPY SUPPRESSOR OF IRA1 4 (MSI4)/FVE, is involved in non-CG methylation at several RdDM targets, suggesting a role of FVE in RdDM. However, whether and how FVE affects RdDM genome-wide is not known. Here, we report that FVE is required for DNA methylation at thousands of RdDM target regions. In addition, dysfunction of FVE significantly reduces 24-nucleotide siRNA accumulation that is dependent on factors downstream in the RdDM pathway. By using chromatin immunoprecipitation and sequencing (ChIP-seq), we show that FVE directly binds to FVE-dependent 24-nucleotide siRNA cluster regions. Our results also indicate that FVE may function in RdDM by physically interacting with RDM15, a downstream factor in the RdDM pathway. Our study has therefore revealed that FVE, by associating with RDM15, directly regulates DNA methylation and siRNA accumulation at a subset of RdDM targets.
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