期刊
PLANT DISEASE
卷 106, 期 1, 页码 182-189出版社
AMER PHYTOPATHOLOGICAL SOC
DOI: 10.1094/PDIS-05-21-1046-RE
关键词
Diaporthe eres; molecular phylogenetic analysis; pathogenicity; peach shoot blight; Phomopsis liquidambaris
资金
- National Natural Science Foundation of China [31101475]
- Natural Science Foundation of Jiangsu Province of China [BK20200929]
- Yangzhou University Research Foundation for Advanced Talents [5018/137010407, 5018/137011834]
- China Agriculture Research System of Ministry of Finance (MOF) [CARS-30-3-02]
- China Agriculture Research System of Ministry of Agriculture and Rural Affairs of the People's Republic of China (MARA) [CARS-30-3-02]
- Pilot Project of Agricultural Major Technology Collaborative Popularization Plan [(18) 003]
Peach shoot blight (PSB) has caused significant production loss in the peach industry in China. The study identified Phomopsis amygdali as the common causal agent, and also discovered two new pathogens, Phomopsis liquidambaris and Diaporthe eres, that could cause PSB. It was found that Phomopsis liquidambaris and Diaporthe eres might become dominant species in the future, highlighting the importance of developing effective disease control strategies.
Peach shoot blight (PSB), which kills shoots, newly sprouted leaf buds, and peach fruits, has gradually increased over the last 10 years and resulted in 30 to 50% of total production loss of the peach industry in China. Phomopsis amygdali has been identified as the common causal agent of this disease. In this study, two new species, Phomopsis liquidambaris (strain JW18-2) and Diaporthe eres (strain JH18-2), were also pathogens causing PSB, as determined through molecular phylogenetic analysis based on the sequences of the internal transcribed spacer (ITS) region, translation elongation factor 1-alpha (EF1-alpha) and beta-tubulin (TUB), and colony and conidial morphological characteristics. Biological phenotypic analysis showed that the colony growth rate of strain JW18-2 was faster than that of strains JH18-2 and ZN32 (one of the P. amygdali strains that we previously found and identified). All three strains produced alpha-conidia; however, JW18-2 could not produce beta-conidia on alfalfa decoction and Czapek media, and the beta-conidia produced by strain JH18-2 were shorter in length and thicker in width than those produced by strain ZN32. Pathogenicity tests showed that JW18-2 presented the strongest pathogenicity for peach fruits and twigs and was followed by strains JH18-2 and ZN32. The results shed light on the etiology of PSB and provide a warning that P. liquidambaris or D. eres might develop into dominant species after a few years while also potentially benefitting the development of effective disease control management strategies.
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