期刊
PHYTOTHERAPY RESEARCH
卷 35, 期 11, 页码 6270-6280出版社
WILEY
DOI: 10.1002/ptr.7272
关键词
Licochalcone A; mast cells; MRGPRX2; NF-kappa B; pseudo-allergy
资金
- China Postdoctoral Science Foundation [2019M653672]
- Fundamental Research Funds for the Central University [xjj2018168]
Lico A demonstrates anti-pseudo-allergy effects by inhibiting MRGPRX2-mediated pseudo-allergic reactions through blocking NF-kappa B nuclear migration.
Licochalcone A (Lico A) is a natural flavonoid belonging to the class of substituted chalcone that has various biological effects. Mast cells (MCs) are innate immune cells that mediate hypersensitivity and pseudo-allergic reactions. MAS-related GPR family member X2 (MRGPRX2) on MCs has been recognized as the main receptor for pseudo-allergic reactions. In this study, we investigated the anti-pseudo-allergy effect of Lico A and its underlying mechanism. Substance P (SP), as an MC activator, was used to establish an in vitro and in vivo model of pseudo-allergy. The in vivo effect of Lico A was investigated using passive cutaneous anaphylaxis (PCA) and active systemic allergy, along with degranulation, Ca2+ influx in vitro. SP-induced laboratory of allergic disease 2 (LAD2) cell mRNA expression was explored using RNA-seq, and Lico A inhibited LAD2 cell activation by reverse transcription polymerase chain reaction (RT-PCR), western blotting, and immunofluorescence staining. Lico A showed an inhibitory effect on SP-induced MC activation and pseudo-allergy both in vitro and in vivo. The nuclear factor (NF)-kappa B pathway is involved in MRGPRX2 induced MC activation, which is inhibited by Lico A. In conclusion, Lico A inhibited the pseudo-allergic reaction mediated by MRGPRX2 by blocking NF-kappa B nuclear migration.
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