Neuroprotective effects of salidroside on ageing hippocampal neurons and naturally ageing mice via the PI3K/Akt/TERT pathway

Studies have found that salidroside, isolated from Rhodiola rosea L, has various pharmacological activities, but there have been no studies on the effects of salidroside on brain hippocampal senescence. The purpose of this study was to investigate the mechanistic role of salidroside in hippocampal neuron senescence and injury. In this study, long-term cultured primary rat hippocampal neurons and naturally aged C57 mice were treated with salidroside. The results showed that salidroside increased the viability and MAP2 expression, reduced beta-galactosidase (beta-gal) levels of rat primary hippocampal neurons. Salidroside also improved cognition dysfunction in ageing mice and alleviated neuronal degeneration in the ageing mice CA1 region. Moreover, salidroside decreased the levels of oxidative stress and p21, p16 protein expressions of hippocampal neurons and ageing mice. Salidroside promoted telomerase reverse transcriptase (TERT) protein expression via the phosphatidylinositol-3-kinase (PI3K)/protein kinase B (Akt) pathway. In conclusion, our findings suggest that salidroside has the potential to be used as a therapeutic strategy for anti-ageing and ageing-related disease treatment.

Automated summary

Salidroside isolated from Rhodiola rosea L has been found to have potential therapeutic effects on hippocampal neuron senescence and injury. It can increase cell viability, reduce beta-galactosidase levels, improve cognition dysfunction in ageing mice, and alleviate neuronal degeneration in the CA1 region of ageing mice. Additionally, salidroside may exert its protective effects through reducing oxidative stress levels, inhibiting p21 and p16 protein expressions, and promoting telomerase reverse transcriptase protein expression.