期刊
PHYTOMEDICINE
卷 89, 期 -, 页码 -出版社
ELSEVIER GMBH
DOI: 10.1016/j.phymed.2021.153615
关键词
Polysaccharide; Inflammation; Lipopolysaccharide; Macroalgae
资金
- UGC-SAP
- DST-FIST
- UGC
The study showed that a sulfated polysaccharide extracted from marine algae has significant immunomodulatory effects in experimental rats, reducing systemic inflammation and possessing antioxidant and anti-inflammatory potential.
Background: Inflammation and oxidative stress are common pathologies in a wide range of chronic diseases. Polysaccharides are known to exhibit antioxidant and anti-inflammatory potential and are suggested to possess immunomodulatory potential. Purpose: Herein, the immunomodulatory activity of a sulfated polysaccharide (PS) separated from a brown marine algae Turbinaria ornata is studied in LPS instigated systemic inflammation in experimental rats. Study design and methods: Male SD rats are pretreated with different doses of PS (2.5, 5, 10 mg/kg bw) for a week followed by inducing systemic inflammation using LPS (10 mg/kg i.p.). Blood withdrawn after 8 h of LPS injection is subjected to hematological analysis (WBC, HCT, and PLT). After 24 h of LPS induction, cardiac tissue was isolated and subjected to biochemical, molecular, and histopathological analysis. Effect of PS pre-treatment (2.5, 5, 10 mg/kg bw) was checked by assessing serum parameters (AST, CK-MB, and gamma GT), antioxidant markers (LPO, GSH, SOD, Grx) and inflammatory markers (IL1 beta, IL6, IL10, NF kappa B), followed by analyzing the iNOS, PI3k and Akt to identify the probable mode of action. Results: Elevated levels of AST, CK-MB, and gamma GT in serum were significantly reduced on PS pretreatment. LPS significantly raised the LPO and Grx levels in heart tissue whereas, PS pre-treatment significantly reduced LPO and Grx levels. GSH and SOD levels were reduced upon LPS induction and were brought to near normal by HD of PS. PS also reduced the mRNA levels of IL6, Trx, and increased IL10 levels in the heart tissue substantiating its anti-inflammatory and antioxidant potency. Further, IL1 beta, NF kappa B, iNOS, and pPI3k/pAkt expressions were significantly modulated by PS in the cardiac tissue substantiating the immunomodulatory effect. A trend of improvement in the inflammatory pathology was also observed in the heart tissue compared to LPS control, as confirmed by histopathology analysis. Conclusion: Altogether, this study concludes the immunomodulatory potential of PS from the marine macroalgae Turbinaria ornata significantly and prevents LPS induced systemic inflammation in the cardiac tissue presumably influenced by the glucopyranose and fucopyranose subunits in the polysaccharide.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据