期刊
PHYTOCHEMISTRY
卷 189, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phytochem.2021.112816
关键词
Talaromyces stipitatus; Trichocomaceae; Steroids; Cytotoxicity
资金
- Program for Changjiang Scholars of Ministry of Education of the People's Republic of China [T2016088]
- National Natural Science Foundation for Distinguished Young Scholars [81725021]
- Tongji-Rongcheng Center for Biomedicine, Huazhong University of Science and Technology
- National Natural Science Foundation for Excellent Young Scholars [81922065]
- National Science and Technology Project of China [2018ZX09201001-001003]
- Innovative Research Groups of the National Natural Science Foundation of China [81721005]
- National Natural Science Foundation of China [82003633]
- Academic Frontier Youth Team of HUST [2017QYTD19]
- Integrated Innovative Team for Major Human Diseases Program of Tongji Medical College (HUST)
Five undescribed sterol derivatives were isolated from Talaromyces stipitatus, along with eight known congeners, marking the first example of this class of ergosterols from T. stipitatus. The compounds were tested for their effects on hepatoma cell lines, with two of them further evaluated for their impacts on cell cycle progression and apoptosis due to their pronounced cytotoxicity. The antiproliferative activities were mainly mediated by inducing cell apoptosis, according to the research results.
Five undescribed sterol derivatives, (22E,24R)-7 alpha-methoxy-5 alpha,6 alpha-epoxyergosta-8(14),22-diene-3 beta,15 beta-diol, (22E,24R)-5 alpha,6 alpha-epoxyergosta-8(14),22-diene-3 beta,7 beta,15 alpha-triol, (22E,24R)-3 beta,5 alpha-dihydroxy-14 beta,15 beta-epoxyergosta-7,22-diene-6-one, (22E,24R)-6 alpha-methoxy-7 alpha,15 beta-dihydroxyergosta-4,8(14),22-triene-3-one, and (25S)ergosta-7,24(28)-diene-3 beta,4 alpha,6 alpha,26-tetraol were isolated from the extract of Talaromyces stipitatus, along with eight known congeners. This is the first example of a class of ergosterols isolated from T. stipitatus. Their structures with absolute configurations were elucidated based on NMR spectroscopic data, ECD calculations, and X-ray crystallographic analyses. All these compounds were tested for their effects on three hepatoma cell lines including Hep3B, HepG2, and Huh-7. Moreover, (22E,24R)-5 alpha,6 alpha-epoxyergosta-8(14),22-diene-3 beta,7 beta,15 alpha-triol and (22E,24R)-9 alpha,15 alpha-dihydroxyergosta-4,6,8(14),22-tetraen-3-one were further evaluated for their impacts on cell cycle progression and apoptosis due to their pronounced cytotoxicity, to uncover their underlying mechanisms. Our results suggested that their antiproliferative activities were mainly mediated by inducing cell apoptosis.
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