期刊
PARASITOLOGY RESEARCH
卷 120, 期 10, 页码 3537-3546出版社
SPRINGER
DOI: 10.1007/s00436-021-07284-8
关键词
Babesia spp; Capybara; Opossum; Rodents
类别
资金
- FAPESP (Foundation for Research Support of the State of Sao Paulo) [2018/02753-0, 2020/12037-0]
- FUNDECT (Foundation for Support to the Development of Education, Science and Technology of the State of Mato Grosso do Sul) [59/300.187/2016]
- CNPq (National Council for Scientific and Technological Development) [302420/2017-7, 308768/2017-5]
- CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior)
The study detected and characterized piroplasmids from wild mammals and associated ticks in Central-Western Brazil, revealing a potential novel piroplasmid species and identifying partial 18S rRNA gene sequences related to piroplasms found in capybaras in rodents.
The order Piroplasmida encompasses tick-borne pathogens of veterinary and medical importance positioned in two main families: Babesiidae and Theileriidae. Even though previous studies carried out in Brazil recorded the occurrence of piroplasmid species circulating in small mammals, 18S RNA gene sequences were only partially sequenced, preventing the assessment of their phylogenetic positioning. The current study aimed to detect and characterize, using morphological, molecular, and bioinformatic approaches, piroplasmids from wild mammals and associated ticks sampled in Central-Western Brazil. Out of 67 Didelphis albiventris sampled, 22 (16.4%) were positive for piroplasmids by PCR. In contrast, none of the 48 small rodents and 14 capybaras (Hydrochoerus hydrochaeris) was PCR-positive. Four Amblyomma dubitatum ticks-one from Rattus rattus, one from H. hydrochaeris, and two from D. albiventris-out of 114 Amblyomma spp. DNA samples were positive for piroplasmids by PCR. The phylogenetic inference performed using the near-complete 18S rRNA gene positioned the putative novel piroplasmid species detected in D. albiventris and associated A. dubitatum ticks near to Babesia sensu lato clade (Western group-cluster III) and distant from the Australian marsupial-associated piroplasms. Phylogenetic inferences based on two additional molecular markers, namely hsp-70 and cox-1, supported the near-complete 18S rRNA gene phylogenetic inference. Finally, the partial 18S rRNA gene sequences detected in ticks from rodents (R. rattus and H. hydrochaeris) showed 97.2-99.4% identity with the Piroplasmida previously detected in a capybara from Brazil, raising evidence that a still uncharacterized piroplasmid species has been identified in the capybara, the largest rodent species from South America.
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