期刊
PARASITOLOGY INTERNATIONAL
卷 83, 期 -, 页码 -出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.parint.2021.102346
关键词
Human schistosomiasis; Schistosoma mansoni; Transmission monitoring; Antibody detection; Serpin; RP26
类别
资金
- Global Health Innovative Technology Fund [T2017-272]
- JSPS [17H01684]
- Grants-in-Aid for Scientific Research [17H01684] Funding Source: KAKEN
In this study, the detection of antibodies against recombinant protein antigens Schistosoma mansoni serine protease-inhibitor (SmSerpin) and RP26 showed high sensitivity and specificity in plasma samples, indicating an effective method for detecting light-intensity infections of S. mansoni.
Schistosomiasis remains a worldwide public health problem, especially in sub-Saharan Africa. The World Health Organization targets the goal for its elimination as a public health problem in the 2030 Neglected Tropical Diseases (NTDs) Roadmap. Concerted action and agile responses to challenges will be necessary to achieve the targets. Better diagnostic tests can accelerate progress towards the elimination by monitoring disease trends and evaluating the effectiveness of interventions; however, current examinations such as Kato-Katz technique are of limited power to detect light-intensity infections. The point-of-care circulating cathodic antigen (POC-CCA) test shows a higher sensitivity compared to the reference standard, Kato-Katz technique, but it still lacks sufficient sensitivity with low infection intensity. In this study, we examined antibody reactions against recombinant protein antigens; Schistosoma mansoni serine protease-inhibitor (SmSerpin) and RP26, by enzyme-linked immunosorbent assay (ELISA) in plasma samples with light-intensity infection. The sensitivity using the cocktail antigen of recombinant SmSerpin and RP26 showed 83.7%. The sensitivity using S. mansoni soluble egg antigen (SmSEA) was 90.8%, but it showed poor specificity (29.7%), while the cocktail antigen presented improved specificity (61.4%). We conclude that antibody detection to the SmSerpin and RP26 protein antigens is effective to detect S. mansoni light-intensity infections. Our study indicates the potential of detecting antibody against recombinant protein antigens to monitor the transmission of schistosomiasis in low endemicity contexts.
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