期刊
PARASITOLOGY
卷 148, 期 12, 页码 1475-1481出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0031182021001074
关键词
Cell-free culture; gametes; gametogenesis; gamonts; Isospora suis; qPCR
类别
资金
- Graduate School `Pig and Poultry Medicine' of the University of Veterinary Medicine Vienna
- Austrian Science fund (FWF) [P 33123]
Coccidia, including Cystoisospora suis, exhibit a characteristic life cycle involving a switch between asexual and sexual development. A novel host cell-free cultivation method allows for the completion of the C. suis life cycle in vitro, providing a new tool for detailed research and potential evaluation of drug or vaccine targets.
Coccidia display a characteristic life cycle, where the parasites switch between asexual and sexual development, resulting in an environmental stage, the oocyst. The entero-pathogenic Cystoisospora suis, a coccidian parasite of swine and close relative to Toxoplasma gondii, undergoes development in one host-cycle. Despite the well-described intracellular development of Coccidia, the C. suis life cycle can progress in an in vitro, host cell-free system after initial intracellular development of merozoites. A novel host cell-free cultivation method was developed by transferring purified merozoites from cell culture supernatant (dpi 6) to culture medium and incubating them for 5 days to induce their progression to sexually differentiated stages. The development of sexual stages in the absence of host cells was verified by morphological studies, flow cytometry and the transcription analysis of three genes linked to sexual stages (HAP2, OWP and TyRP). The host cell-free culture permits the sexual development (and with this, the complete life cycle progression from sporozoites to oocysts) of C. suis in vitro and provides a new tool for detailed research on the development of C. suis and possibly other Coccidia. This will also be useful for the evaluation of novel drug or vaccine targets in these parasites.
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