Morphological and molecular characterization of Quinqueserialis (Digenea: Notocotylidae) species diversity in North America

Estimates of trematode diversity are inaccurate due to unrecognized cryptic species and phenotypic plasticity within species. Integrative taxonomy (genetics, morphology and host use) increases the clarity of species delineation and improves knowledge of parasite biology. In this study, we used this approach to resolve taxonomic issues and test hypotheses of cryptic species in a genus of trematode, Quinqueserialis. Specimens from throughout North America were field collected from hosts and obtained from museums. We found three morphologically distinct groups and successfully sequenced specimens from two of these groups. DNA sequencing at the 28S and CO1 gene regions revealed that two of the three groups were genetically distinct. One genetic group included two morphological clusters demonstrating host-induced phenotypic plasticity within Quinqueserialis quinqueserialis. The other unique genetic group is a novel species, Quinqueserialis kinsellai n. sp., which is described herein. Our study illustrates the importance of integrating multiple sources of evidence when investigating trematode diversity to account for the influence of cryptic species or phenotypic plasticity. However, further sampling is needed to understand Quinqueserialis spp. diversity as some species have no genetic information associated with them.

Automated summary

Estimates of trematode diversity are inaccurate due to unrecognized cryptic species and phenotypic plasticity within species. However, integrative taxonomy, as demonstrated in this study of the genus Quinqueserialis, can help resolve taxonomic issues and uncover new species. Further sampling is needed to fully understand the diversity of Quinqueserialis spp. and account for species with no associated genetic information.