期刊
ORAL ONCOLOGY
卷 117, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.oraloncology.2021.105270
关键词
HCCC; IGF2; EWSR1; ATF1; CXCR4
资金
- NIH [T32 DC005356, U01-DE025184, R01CA194536]
This study conducted full characterization of five independent HCCC cases, revealing insulin-like growth factor alterations and identifying a 354 gene signature that can differentiate HCCC from MEC. The study also discovered elevated expression of ATF1 binding motif-enriched genes in HCCC, supporting a transcriptional pathogenic mechanism related to EWSR1-ATF1 fusion. Furthermore, analysis showed elevated levels of IGF1R, SGK1, SGK3, and CXCR4 protein in HCCC cases, potentially providing actionable alterations for clinical care.
Objectives: Salivary gland tumors are comprised of a diverse group of malignancies with widely varying prognoses. These cancers can be difficult to differentiate, especially in cases with limited potential for immunohistochemistry (IHC)-based characterization. Here, we sought to define the molecular profile of a rare salivary gland cancer called hyalinizing clear cell carcinoma (HCCC), and identify a molecular gene signature capable of distinguishing between HCCC and the histopathologically similar disease, mucoepidermoid carcinoma (MEC). Materials and methods: We performed the first integrated full characterization of five independent HCCC cases. Results: We discovered insulin-like growth factor alterations and aberrant IGF2 and/or IGF1R expression in HCCC tumors, suggesting a potential dependence on this pathway. Further, we identified a 354 gene signature that differentiated HCCC from MEC, and was significantly enriched for genes with an ATF1 binding motif in their promoters, supporting a transcriptional pathogenic mechanism of the characteristic EWSR1-ATF1 fusion found in these tumors. Of the differentially expressed genes, IGF1R, SGK1 and SGK3 were found to be elevated in the HCCCs relative to MECs. Finally, analysis of immune checkpoints and subsequent IHC demonstrated that CXCR4 protein was elevated in several of the HCCC cases. Conclusion: Collectively, our data identify an ATF1-motif enriched gene signature that may have clinical utility for molecular differentiation of HCCCs from other salivary gland tumors and discover potential actionable alterations that may benefit the clinical care of recurrent HCCC patients.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据