期刊
OPTICS LETTERS
卷 46, 期 15, 页码 3588-3591出版社
OPTICAL SOC AMER
DOI: 10.1364/OL.428193
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- High-level Talents of Xiamen University of Technology [YKJ19010R]
The dual-axis confocal configuration offers high axial resolution and long working distance for depth-resolved fluorescence spectroscopy. By using a dual-axis illumination configuration, it is possible to locate the target layer with high resolution and collect fluorescence spectra from microscopic regions.
The dual-axis confocal (DAC) configuration provides a high axial resolution, long working distance (WD), and large dynamic range. These properties can reveal depth-resolved fluorescence spectra. We present a depth sensitive fluorescence spectroscopy based on the DAC configuration. The system enables high axial resolution of 3.23 mu m and a long WD of 3.73 mm compared to that of 4.68 mu m and 2.1 mm for comparable single-axis confocal configurations, respectively. Besides, a DAC configuration also offers a superior dynamic range and rejection of out-of-focus scattered light based on the principle of Huygens-Fresnel integrals. Additionally, to locate the target layer, the collection path of the DAC configuration will be used as the other illumination path, forming a dual-axis illumination configuration. These beam paths are used to locate the target layer using a white light imaging system with a commercial lownumerical aperture objective. A multi-layer fluorescence phantom of Barrett's esophagus containing fluorescein isothiocyanate and Alexa Fluor 514 was used to verify the principle of depth-resolved fluorescence spectroscopy. The results show that the DAC configuration can collect fluorescence spectra from microscopic regions with high axial resolution. (C) 2021 Optical Society of America
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