4.6 Article

High-speed Fourier ptychographic microscopy for quantitative phase imaging

期刊

OPTICS LETTERS
卷 46, 期 19, 页码 4785-4788

出版社

OPTICAL SOC AMER
DOI: 10.1364/OL.428731

关键词

-

类别

资金

  1. Innovation and Technology Commission [ITS/394/17]
  2. Croucher Foundation [CM/CT/CF/CIA/0688/19ay]

向作者/读者索取更多资源

This study introduces and demonstrates a high-speed FPM method based on laser illumination and digital micro-mirror devices for illumination angle scanning. With this method, quantitative phase imaging and intensity imaging can be achieved at over 42 frames per second with approximately 1 micron lateral resolution, enabling the visualization of membrane height fluctuations in red blood cells and subcellular features in stained cancer tissue slices.
Fourier ptychographic microscopy (FPM), as an emerging computational imaging method, has been applied to quantitative phase imaging with resolution bypassing the physical limit of the detection objective. Due to the weak illumination intensity and long image acquisition time, the achieved imaging speed in current FPM methods is still low, making them unsuitable for real-time imaging applications. We propose and demonstrate a high-speed FPM method based on using laser illumination and digital micro-mirror devices for illumination angle scanning. In this new, to the best of our knowledge, FPM method, we realized quantitative phase imaging and intensity imaging at over 42 frames per second (fps) with around 1 mu m lateral resolution. The quantitative phase images have revealed membrane height fluctuations of red blood cells with nanometer-scale sensitivity, while the intensity images have resolved subcellular features in stained cancer tissue slices. (C) 2021 Optical Society of America

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据