Pinosylvin inhibits migration and invasion of nasopharyngeal carcinoma cancer cells via regulation of epithelial-mesenchymal transition and inhibition of MMP-2

Nasopharyngeal carcinoma (NPC) is a tumor located in the nasopharynx with highly invasive and metastatic properties. Metastasis is a primary cause of mortality in patients with NPC. The terpenoid polyphenol pinosylvin is a known functional compound of the Pinus species that exhibits anti-inflammatory effects; however, the effect of pinosylvin on human NPC cell migration and invasion is unclear. The present study aimed to investigate the functional role of pinosylvin in NPC cells (NPC-039, NPC-BM and RPMI 2650). Gap closure and Transwell assay indicated that pinosylvin at increasing concentrations inhibited migration and invasion of NPC-039 and NPC-BM cells. In addition to inhibiting the enzyme activity of MMP-2, pinosylvin also decreased the protein expression levels of MMP-2 and MMP-9. Pinosylvin decreased the expression of vimentin and N-cadherin and significantly increased the expression of zonula occludens-1 and E-cadherin in NPC cells. Additionally, pinosylvin suppressed the invasion and migration ability of NPC-039 and NPC-BM cells by mediating the p38, ERK1/2 and JNK1/2 pathways. The present results revealed that pinosylvin inhibited migration and invasion in NPC cells.

Automated summary

The study demonstrated that pinosylvin inhibited migration and invasion in nasopharyngeal carcinoma cells, potentially through modulation of multiple signaling pathways.