Long non-coding RNA H19 regulates LASP1 expression in osteosarcoma by competitively binding to miR-29a-3p

A prevalent type of bone tumor, osteosarcoma (OS) is prone to pulmonary metastasis, which results in a high relapse risk and poor prognosis for patients. The progression of OS is significantly associated with the expression of long non-coding (lnc)RNA H19. To the best of our knowledge, however, the exact molecular mechanism of this lncRNA has not been fully investigated. The present study verified the effect of H19 on the proliferation and invasion of osteosarcoma cells via in vivo and in vitro experiments, including Cell Counting Kit-8, western blot, reverse transcription-quantitative PCR, wound healing and Transwell assays. H19 was found to be overexpressed in OS compared with corresponding normal adjacent tissue. In addition, H19 served as a competing endogenous ncRNA targeting microRNA-29a-3p and activating LIM and SH3 domain protein 1 and modulating the OS cell phenotype. The results of the present study may improve understanding of OS pathogenesis.

Automated summary

Osteosarcoma, a prevalent type of bone tumor, is prone to pulmonary metastasis, leading to high relapse risk for patients. The overexpression of H19 is significantly associated with the progression of osteosarcoma, influencing tumor cell phenotype by regulating microRNA-29a-3p and LIM and SH3 domain protein 1. This study may contribute to a better understanding of osteosarcoma pathogenesis.