4.8 Article

Targeting HIF-1 alpha transcriptional activity drives cytotoxic immune effector cells into melanoma and improves combination immunotherapy

期刊

ONCOGENE
卷 40, 期 28, 页码 4725-4735

出版社

SPRINGERNATURE
DOI: 10.1038/s41388-021-01846-x

关键词

-

资金

  1. Luxembourg Institute of Health
  2. FNRS Televie [7.4535.16, 7.6505.18, 7.4606.18]
  3. Luxembourg National Research Fund [C18/BM/12670304/COMBATIC, PRIDE15/10675146/CANBIO]
  4. Fondation Cancer, Luxembourg [FC/2018/06]
  5. Kriibskrank Kanner Foundation, Luxembourg (2019)
  6. Janssen Cilag Pharma
  7. Roche Pharma, Action LIONS Vaincre le Cancer Luxembourg
  8. Sheik Hamdan Bin Rashid Al Maktoum Foundation, United Arab Emirates

向作者/读者索取更多资源

Inhibition of HIF-1 alpha transcriptional activity can increase levels of NK and CD8(+) T cells in the tumor microenvironment, enhancing the efficacy of cancer immunotherapy. Combining acriflavine can significantly improve the benefits of cancer immunotherapy based on TRP-2 peptide vaccination and anti-PD-1 blocking antibody, while melanoma patients with high CCL5 levels have a higher survival rate.
Hypoxia is a key factor responsible for the failure of therapeutic response in most solid tumors and promotes the acquisition of tumor resistance to various antitumor immune effectors. Reshaping the hypoxic immune suppressive tumor microenvironment to improve cancer immunotherapy is still a relevant challenge. We investigated the impact of inhibiting HIF-1 alpha transcriptional activity on cytotoxic immune cell infiltration into B16-F10 melanoma. We showed that tumors expressing a deleted form of HIF-1 alpha displayed increased levels of NK and CD8(+) effector T cells in the tumor microenvironment, which was associated with high levels of CCL2 and CCL5 chemokines. We showed that combining acriflavine, reported as a pharmacological agent preventing HIF-1 alpha/HIF-1 beta dimerization, dramatically improved the benefit of cancer immunotherapy based on TRP-2 peptide vaccination and anti-PD-1 blocking antibody. In melanoma patients, we revealed that tumors exhibiting high CCL5 are less hypoxic, and displayed high NK, CD3(+), CD4(+) and CD8(+) T cell markers than those having low CCL5. In addition, melanoma patients with high CCL5 in their tumors survive better than those having low CCL5. This study provides the pre-clinical proof of concept for a novel triple combination strategy including blocking HIF-1 alpha transcription activity along vaccination and PD-1 blocking immunotherapy.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.8
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据