期刊
ONCOGENE
卷 40, 期 29, 页码 4820-4831出版社
SPRINGERNATURE
DOI: 10.1038/s41388-021-01908-0
关键词
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资金
- Natural Science Foundation of Henan Province [182300410361]
- Major Project of Science and Technology in Henan Province [161100311400]
- National Science and Technology Major Project of China [2018ZX10302205]
- Project of Basic Research Fund of Henan Institute of Medical and Pharmacological Sciences [2020BP0107, 2020BP0111]
- Zhengzhou Major Project for Collaborative Innovation [18XTZX12007]
- Key Scientific and Technological Project of Henan Province [212102310124]
This study analyzed HCC tumor and adjacent normal tissue samples using TMT-labeled tandem mass spectrometry and whole-transcriptome sequencing, identifying 977 differentially expressed proteins and 243 genes, and comprehensively evaluating gene-regulatory circuits in HCC. Bioinformatics analysis revealed involvement of certain miRNAs in regulating hub genes, while crucial coexpression and competing endogenous RNA interaction axes revealed potential functions of lncRNAs and circRNAs in HCC.
Hepatocellular carcinoma (HCC) is the most common subtype of primary liver cancer and one of the leading causes of cancer-related death worldwide. To gain more insights into the transcriptomic landscape and molecular mechanism of HCC, we performed TMT-labelled tandem mass spectrometry (n = 4) and whole-transcriptome sequencing (n = 3) based on HCC tumour (T) and adjacent normal (N) tissues from seven HCC patients. To comprehensively evaluate the gene-regulatory circuits in HCC, differential expression and enrichment analyses were performed on the differentially expressed proteins (DEPs), genes (DEGs), miRNAs (555), lncRNAs (29) and circRNAs (895). A total of 977 proteins and 243 genes were found to be differentially expressed in HCC tumours compared with adjacent normal tissues. HCC data from The Cancer Genome Atlas were used to validate the results. Combined with the results above, 56 DEP-DEGs with common changes in relative quantity were identified. Functional pathway analysis showed that the DEP-DEGs were mainly enriched in the spliceosome and various metabolic processes. Bioinformatics analysis showed that hsa-miR-1266-5p, hsa-miR-128-1-5p, hsa-miR-139-5p, hsa-miR-34b-3p and hsa-miR-570-3p were involved in the regulation of the hub genes mentioned above. The crucial coexpression (lncRNA-mRNA, circRNA-mRNA) and competing endogenous RNA interaction axes showed the possible functions of the lncRNAs and circRNAs. We explored potential cancer biomarkers by combining proteomic and transcriptomic studies. Our study provides a valuable resource for understanding regulatory mechanisms at the RNA level and may ultimately further assist in the development of diagnostic and/or therapeutic targets for HCC.
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