Advancement in specific strand scission of DNA and evaluation of in-vitro biological assessment by pharmacologically significant tetraaza macrocyclic metal complexes constrained by triazole

A novel ligand, tetra-azamacrocyclic, L(C24H16N12O2S4) and its complexes with the structures, [MLCl2] and [CuL]Cl-2 (where M = Ni(II), Fe(II); L = S,S'-[benzene-1,3-diylbis(4H-1,2,4-triazole-5,3diyl)]bis{[(5-benzene-1,3-diyl-4H-1,2,4-triazol-3yl)sulfanyl] ethanethioate} were described and depicted by the analytical and spectral techniques. These studies revealed an octahedral geometry for Ni(II) and Fe(II) structures, while the Cu(II) complex exhibited a square planar structure. All the integrated metal structures were screened against targeted species of pathogenic fungi and bacterial strains to evaluate in vitro antimicrobial activities. The binding capability of the complexes with CT-DNA was considered by supporting detailed examination followed by viscosity measurements and thermal denaturation studies. The mode of DNA cleavage using gel electrophoreses revealed that the complexes possess photonuclease characteristics against pUC19 DNA under UV-visible irradiation. The newly synthesized novel Cu(II)-L complex has been proven to be a potential DNA cleavage agent and a potent inhibitor against both the bacterial and fungi strains. [GRAPHICS]

Automated summary

This study describes the synthesis and structures of a novel ligand and its metal complexes, along with their in vitro antimicrobial activities and interaction with DNA. The results revealed potential DNA cleavage and inhibitory properties of the newly synthesized Cu(II)-L complex against both bacterial and fungal strains.