期刊
NUCLEIC ACIDS RESEARCH
卷 49, 期 13, 页码 7375-7388出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkab530
关键词
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资金
- National Research Foundation, Singapore [NRF-RF201010]
- Singapore Ministry of Health's National Medical Research Council [NMRC/CIRG/1467/2017, NMRC/CIRG/1358/2013]
- Genome Institute of Singapore (GIS)/Agency for Science, Technology and Research (A*STAR)
- Singapore National Medical Research Council
The study revealed that three non-phasevariable Type I RMSs in Escherichia coli have no measurable impact on gene expression, in vivo virulence, or any of 1190 in vitro growth phenotypes. This suggests that there may be other nonregulatory methylation systems like these, expanding our understanding of the potential role of RMSs in bacterial evolution.
DNA methylation is a common epigenetic mark that influences transcriptional regulation, and therefore cellular phenotype, across all domains of life. In particular, both orphan methyltransferases and those from phasevariable restriction modification systems (RMSs) have been co-opted to regulate virulence epigenetically in many bacteria. We now show that three distinct non-phasevariable Type I RMSs in Escherichia coli have no measurable impact on gene expression, in vivo virulence, or any of 1190 in vitro growth phenotypes. We demonstrated this using both Type I RMS knockout mutants as well as heterologous installation of Type I RMSs into two E. coli strains. These data provide three clear and currently rare examples of restriction modification systems that have no impact on their host organism's gene regulation. This leads to the possibility that other such nonregulatory methylation systems may exist, broadening our view of the potential role that RMSs may play in bacterial evolution.
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