期刊
NUCLEIC ACIDS RESEARCH
卷 49, 期 16, 页码 9246-9263出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkab679
关键词
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资金
- Natural Science Foundation of China [82073067, 81621004, 81872140, 81872306, 81772532]
- Guangdong Science and Technology Department [2021A0505030084, 2019B020226003]
The study identifies DLX5 as a significant SCC-promoting factor, showing a distinct bivalent reconfiguration of its promoter in normal tissues versus cancer, where it is co-regulated by SOX2 in ESCC. Silencing of DLX5 significantly inhibits SCC viability and collaborates with TP63 to regulate thousands of enhancers and promoters, activating cancer-promoting pathways.
To reconstruct systematically hyperactive transcription factor (TF)-dependent transcription networks in squamous cell carcinomas (SCCs), a computational method (ELMER) was applied to 1293 pan-SCC patient samples, and 44 hyperactive SCC TFs were identified. As a top candidate, DLX5 exhibits a notable bifurcate re-configuration of its bivalent promoter in cancer. Specifically, DLX5 maintains a bivalent state in normal tissues; its promoter is hypermethylation, leading to DLX5 transcriptional silencing in esophageal adenocarcinoma (EAC). In stark contrast, DLX5 promoter gains active histone marks and becomes transcriptionally activated in ESCC, which is directly mediated by SOX2. Functionally, silencing of DLX5 substantially inhibits SCC viability both in vitro and in vivo. Mechanistically, DLX5 co-operates with TP63 in regulating similar to 2000 enhancers and promoters, which converge on activating cancer-promoting pathways. Together, our data establish a novel and strong SCC-promoting factor and elucidate a new epigenomic mechanism - bifurcate chromatin re-configuration - during cancer development.
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