期刊
NUCLEIC ACIDS RESEARCH
卷 49, 期 17, 页码 9906-9925出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkab785
关键词
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资金
- Televie/Fonds National de la Recherche (F.R.S.FNRS)/Fonds National de la Recherche du Luxembourg (FNR) [7.4503.11, 7.4633.16]
- Doctoral School for Systems and Molecular Biomedicine, University of Luxembourg
- FNR (PRIDE grant)
- Ligue Nationale Contre le Cancer
- Canadian Institutes of Health Research Foundation [FDN388879]
In this study, XAB2 was identified as a factor required for resistance to seDSBs induced by the chemotherapeutic drug temozolomide, and it prevents Ku retention and abortive HR at seDSBs induced by temozolomide and camptothecin through a pathway operating parallel to the ATM-CtIP-MRE11 axis. XAB2 depletion results in unproductive RAD51-ssDNA associations, leading to increased NHEJ engagement and genetic instability in S/G2.
Replication-associated single-ended DNA double-strand breaks (seDSBs) are repaired predominantly through RAD51-mediated homologous recombination (HR). Removal of the non-homologous end-joining (NHEJ) factor Ku from resected seDSB ends is crucial for HR. The coordinated actions of MRE11-CtIP nuclease activities orchestrated by ATM define one pathway for Ku eviction. Here, we identify the pre-mRNA splicing protein XAB2 as a factor required for resistance to seDSBs induced by the chemotherapeutic alkylator temozolomide. Moreover, we show that XAB2 prevents Ku retention and abortive HR at seDSBs induced by temozolomide and camptothecin, via a pathway that operates in parallel to the ATM-CtIP-MRE11 axis. Although XAB2 depletion preserved RAD51 focus formation, the resulting RAD51-ssDNA associations were unproductive, leading to increased NHEJ engagement in S/G2 and genetic instability. Overexpression of RAD51 or RAD52 rescued the XAB2 defects and XAB2 loss was synthetically lethal with RAD52 inhibition, providing potential perspectives in cancer therapy. [GRAPHICS] .
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