期刊
NUCLEIC ACIDS RESEARCH
卷 49, 期 13, 页码 7457-7475出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkab526
关键词
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资金
- German Research Foundation (DFG) Grants Project B3 in Research Training Group [GRK 2254]
- German Cancer Aid [70112504]
- International Graduate School of Molecular Medicine, Ulm University
- Alexander von Humboldt Foundation
- Italian Association for Cancer Research (AIRC) [IG ID19797]
- Deutsche Forschungsgemeinschaft, Project B3 in Research Training Group [GRK 2254]
The study reveals distinct DNA replication phenotypes in stem cells and differentiated cells, which are associated with interactions between p53 and POLi proteins. Stem cells slow down DNA replication by promoting complex formation, while differentiated cells synthesize DNA faster, potentially offering therapeutic opportunities focused on DNA replication-based strategies for targeting stem cells.
Using human embryonic, adult and cancer stem cells/stem cell-like cells (SCs), we demonstrate that DNA replication speed differs in SCs and their differentiated counterparts. While SCs decelerate DNA replication, differentiated cells synthesize DNA faster and accumulate DNA damage. Notably, both replication phenotypes depend on p53 and polymerase iota (POLi). By exploring protein interactions and newly synthesized DNA, we show that SCs promote complex formation of p53 and POLi at replication sites. Intriguingly, in SCs the translocase ZRANB3 is recruited to POLi and required for slow-down of DNA replication. The known role of ZRANB3 in fork reversal suggests that the p53-POLi complex mediates slow but safe bypass of replication barriers in SCs. In differentiated cells, POLi localizes more transiently to sites of DNA synthesis and no longer interacts with p53 facilitating fast POLi-dependent DNA replication. In this alternative scenario, POLi associates with the p53 target p21, which antagonizes PCNA poly-ubiquitination and, thereby potentially disfavors the recruitment of translocases. Altogether, we provide evidence for diametrically opposed DNA replication phenotypes in SCs and their differentiated counter-parts putting DNA replication-based strategies in the spotlight for the creation of therapeutic opportunities targeting SCs.
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