Sex differences shape zebrafish performance in a battery of anxiety tests and in response to acute scopolamine treatment

Sex differences influence human and animal behavioral and pharmacological responses. The zebrafish (Danio rerio) is a powerful, popular model system in neuroscience and drug screening. However, the impact of zebrafish sex differences on their behavior and drug responses remains poorly understood. Here, we evaluate baseline anxiety-like behavior in adult male and female zebrafish, and its changes following an acute 30-min exposure to 800-mu M scopolamine, a common psychoactive anticholinergic drug. Overall, we report high baseline anxiety-like behavior and more individual variability in locomotion in female zebrafish, as well as distinct, sex-specific (anxiolytic-like in females and anxiogenic-like in males) effects of scopolamine. Collectively, these findings reinforce the growing importance of zebrafish models for studying how both individual and sex differences shape behavioral and pharmacological responses.

Automated summary

The study found that female zebrafish exhibit higher baseline anxiety-like behavior and more individual variability in locomotion, with sex-specific effects of scopolamine (anxiolytic-like in females and anxiogenic-like in males). These findings highlight the importance of zebrafish models for studying the impact of individual and sex differences on behavioral and pharmacological responses.